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. Author manuscript; available in PMC: 2021 Jun 19.
Published in final edited form as: ACS Synth Biol. 2020 May 7;9(6):1349–1360. doi: 10.1021/acssynbio.0c00038

Figure 4.

Figure 4.

In vitro TT-assay and production of fischerindole. Top: Biosynthetic gene cluster of Fischerella sp. SAG 46.79 (fis). The genes for oxygenases (blue, B1–4), cyclase (pink, C), aromatic prenyltransferases (red, D), GPP synthases (cyan, E1–5), methyl transferase (red, M), tryptophan synthase (magenta, I1–6), isonitrile synthase (green, H1–3) and other core enzymes (black, G1–2) are shown. Bottom: Extracted ion chromatograms (EICs) are shown in all panels. Detection of in vitro synthesized fischerindole products showing EIC (m/z of 305) with no plasmid (A), FamD2 alone (B) for cis-indole isonitrile 3, and FamD2 plus FisC (C) for 12-epi-fischerindole U 8 production. The trace D is the authentic standards of 12-epi-fischerindole U 8.