Table 1.

Proteomics Analysis of FamD2 and FamC1 Expression from in vitro TT-Assay Containing Exogenous Plasmids FamD2 and FamC1, and Substrates 1 and 2 for 12-epi-Hapalindole U 4 Biosynthesis^a

FamD2			FamC1
Total peptide	20		Total peptide	11
Unique peptide	20		Unique peptide	11
Coverage (%)	67.96		Coverage (%)	62.11
Annotated Peptide Sequence	Sequence Positions	PSM FamD2	Annotated Peptide Sequence	Sequence Position	PSM FamC1
[R].IRTDIINVAK.[T]	FamD2 [7–16]	10	[K].RTTWTSNNGVGYVGPGTQFYNQLAPEGR.[N]	FamC1 [70–97]	2
[R].TDIINVAK.[T]	FamD2 [9–16]	6	[R].TTWTSNNGVGYVGPGTQFYNQLAPEGR.[N]	FamC1 [70–97]	8
[K].TFGAEYSEK.[V]	FamD2 [17–25]	3	[R].NIGYIYLSQNPGSGVAGFEQILDATLEPDTK.[Y]	FamC1 [98–128]	35
[K].VLDEVFQVFGEQFADNSFMIR.[T]	FamD2 [26–16]	19	[K].YTLTVDVGNLAGTFK.[G]	FamC1 [129–143]	87
[R].TSNKQPDK.[L]	FamD2 [47–54]	1	[K]. GLSFAGFPGYR. [V]	FamC1 [144–154]	19
[K].LGCYFR.[Y]	FamD2 [55–60]	3	[R].VELLAGDTVLAADHNNLFIK.[E]	FamC1 [155–174]	306
[R].YHEEDESQLGLAWDIAR.[K]	FamD2 [61–77]	4	[K].TSTVTYTSTAK. [D]	FamC1 [180–190]	11
[R].KSGLLSDQGRPVDQLIPEICETFPIMADGVDFDVK.[H]	FamD2 [78–112]	6	[K].DLHLGQK.[L]	FamC1 [191–197]	20
[K].SGLLSDQGRPVDQLIPEICETFPIMADGVDFD VK. [H]	FamD2 [79–112]	8	[R].LVNLLQDK.[F]	FamC1 [202–209]	2
[K].IWQSIK.[G]	FamD2 [118–123]	3	[K].FSGLDFDNVR.[L]	FamC1 [210–219]	15
[K].GWPVQDAFK.[L]	FamD2 [124–133]	5	[R].LVNLLQDKFSGLDFDNVR. [L]	FamC1 [202–219]	2
[K].LSLPASVTTHSDFLK.[N]	FamD2 [134–148]	7
[R].LCFYLPFLNR.[E]	FamD2 [224–233]	2
[R].EAVPQNLLNPLLK.[K]	FamD2 [234–246]	1
[R].EAVPQNLLNPLLKK.[Y]	FamD2 [234–247]	2
[K].KYINEAPALVDNPGFILGWSFGPQGGK.[G]	FamD2 [247–273]	4
[K].YINEAPALVDNPGFILGWSFGPQGGK.[G]	FamD2 [248–273]	2
[K].VDVDYHGR.[T]	FamD2[279–286]	1
[R].TVPLFMK.[V]	FamD2 [287–293]	1
[K].VHSQPLPK.[A]	FamD2 [294–301]	2
FamD2			FamC1
MNDVNRIRTDIINVAKTFGAEYSEKVLDEVFQVFGEQFADNSFMIRTSNKQPDKLGCYFRYHEEDESQLGLAWDIARKSGLLSDQGRPVDQLIPEICETFPIMADGVDFDVKHGLAKIWQSIKGWPVQDAFKLSLPASVTTHSDFLKNHHLDALYAFGIDYHHSSVNLYFDTYHPKHHTSEYYKNLLQDLQFQPPSDELLELLTNNGEIALTFNFASPRIERLCFYLPFLNREAVPQNLLNPLLKKYINEAPALVDNPGFILGWSFGPQGGKGTYTKVDVDYHGRTVPLFMKVHSQPLPKAADFALAQ, 309 residues			MKRKLIVAVWLIFICLGINTPAHATSAVSIPINNAGFENPFMDWDDYTIDTPPGWTTYDPNNLVPEKRTTWTSNNGVGYVGPGTQFYNQLAPEGRNIGYIYLSQNPGSGVAGFEQILDATLEPDTKYTLTVDVGNLAGTFKGLSFAGFPGYRVELLAGDTVLAADHNNLFIKEGEFKTSTVTYTSTAKDLHLGQKLGIRLVNLLQDKFSGLDFDNVRLTTEPTET, 227 residues

^aThe sequence highlighted in yellow represents the coverage of the synthesized protein using proteomic analysis.