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. 2020 Jun 25;12:5049–5056. doi: 10.2147/CMAR.S253412

Figure 3.

Figure 3

Circ-FOXO3 regulated TGFBR2 expression by targeting miR-9-5p. (A and B) The schematic maps of putative binding targets on both miR-9-5p and TGFBR2. (C) Relative miR-9-5p levels in EJ and T24 cells after the transfection of circ-FOXO3 expression vectors. (D) Relative luciferase levels in EJ and T24 cells after the co-transfection of miR-9-5p expression vector and circ-FOXO3 wild type or mutant vector. (E) Relative luciferase levels in EJ and T24 cells after the co-transfection of miR-9-5p expression vector and TGFBR2 wild type or mutant vector. (F) Relative TGFBR2 mRNA levels in EJ and T24 cells after the transfection of circ-FOXO3 expression vector, circ-FOXO3 expression vector with TGFBR2 siRNA or circ-FOXO3 expression vector, TGFBR2 siRNA and TGFBR2 expression vector. (G) Relative TGFBR2 protein levels in EJ and T24 cells after the transfection of circ-FOXO3 expression vector, circ-FOXO3 expression vector with TGFBR2 siRNA or circ-FOXO3 expression vector, TGFBR2 siRNA and TGFBR2 expression vector. **P<0.01.