Figure 1.

Experimental design: Pregnant CD-1 females were exposed to 0 (control), 10 ppb, or 42.5 ppm sodium arsenite in drinking water from E10 to birth. At birth, pups were tossed to females that had not been not exposed to arsenic. Body weight and anogenital distance were measured at E18 the body weight was recorded weekly from 3 to 11 weeks and at 6 months of age. At 8 weeks of age, male pups were included in the fertility study. At 6 months of age, male pups were analyzed for metabolic end points (body composition, glucose test tolerance, serum leptin and insulin levels). At 1 year, sperm samples were collected from the cauda epididymis for quantitative and qualitative analysis.