| Antibodies |
| Anti-iNOS (D6B6S) Rabbit mAb |
Cell Signaling Technology |
Cat# 13120 |
| Anti-PBEF (H-11) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-166946 |
| Anti-NMNAT-2 (B-10) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-515206 |
| Anti-CD38 (H-11) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-374650 |
| Anti-SirT1 (D1D7) Rabbit mAb |
Cell Signaling Technology |
Cat# 9475 |
| Anti-SirT2 (D4S6J) Rabbit mAb |
Cell Signaling Technology |
Cat# 12672 |
| Anti-pADPr (10H) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-56198 |
| Anti-PARP-1 (B-10) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-74470 |
| Anti-β-Actin (C4) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-47778 |
| Anti-GAPDH (G-9) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-365062 |
| Anti-G6PD (G-12) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-373886 |
| Anti-PGD (G-2) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-398977 |
| Anti-IDH1 (F-3) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-515396 |
| Anti-ME1 (C-6) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-365891 |
| Anti-GSR (C-10) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-133245 |
| Anti-FASN (G-11) Mouse mAb |
SANTA CRUZ BIOTECHNOLOGY |
Cat# sc-48357 |
| Anti-MTHFD1 Rabbit pAb |
Proteintech |
Cat# 10794-1-AP |
| Bacterial and Virus Strains |
| BL21(DE3) Chemically Competent Cell |
TRANSGEN BIOTECH |
CD601 |
| Trans5α Chemically Competent Cell |
TRANSGEN BIOTECH |
CD201 |
| Biological Samples |
|
|
| Mouse Primary Cardiomyocytes |
This paper |
N/A |
| Mouse Primary Myoblasts |
This paper |
N/A |
| Human Urine-derived Stem Cells |
Shanghai Jiaotong University affiliated Sixth People’s Hospital |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| NAD+
|
Shanghai YEASEN |
Cat# 60323ES08 |
| NADH |
Shanghai YEASEN |
Cat# 60301ES03 |
| NADP+
|
Shanghai YEASEN |
Cat# 60324ES03 |
| NADPH |
Shanghai YEASEN |
Cat# 60302ES01 |
| ADP |
Shanghai YEASEN |
Cat# 60604ES03 |
| ATP |
Sigma-Aldrich |
Cat# A26209
|
| AMP |
Shanghai YEASEN |
Cat# 60603ES08 |
| Sodium Oxamate |
Sigma-Aldrich |
Cat# O2751 |
| Rotenone |
Sigma-Aldrich |
Cat# R8875 |
| Oligomycin |
Aladdin |
Cat# O111756 |
| Apigenin |
Sigma-Aldrich |
Cat# A3145 |
| FK866 Hydrochloride Hydrate |
Sigma-Aldrich |
Cat# F8557 |
| Nicotinic Acid (NA) |
Sigma-Aldrich |
Cat# N4126 |
| Nicotinamide (NAM) |
Sigma-Aldrich |
Cat# N3376 |
| β-Nicotinamide Mononucleotide (β-NMN) |
Shanghai YEASEN |
Cat# 60303ES80 |
| Nicotinamide Riboside (NR) |
Shanghai Yuanye Bio-technology |
Cat# S24896 |
| Ethyl 3-Aminobenzoate Methanesulfonate (MS-222) |
Sigma-Aldrich |
Cat# E10521
|
| Selisistat (EX 527) |
Selleckchem |
Cat# S1541 |
| Olaparib (AZD2281) |
Selleckchem |
Cat# S1060 |
| AZD2461 |
Selleckchem |
Cat# S7029 |
| 1-Methyl-3-Nitro-1-Nitrosoguanidine (MNNG) |
TCI |
Cat# M0527 |
| 2-Hydroxynicotinic Acid (2-HNA) |
Energy Chemical |
Cat# A010247 |
| Sodium Pyruvate |
Thermo Fisher Scientific |
Cat# 11360070 |
| Lipopolysaccharides (LPS) |
Shanghai YEASEN |
Cat# 60322ES10 |
| Recombinant Mouse IFN-γ |
Absin Bioscience |
Cat# abs01021 |
| Nω-Nitro-L-Arginine Methyl Ester Hydrochloride (L-NAME) |
J&K Scientific |
Cat# 257155 |
| Fugene HD Transfection Reagent |
Promega |
Cat# E2311 |
| Hieff Trans™ Liposomal Transfection Reagent |
Shanghai YEASEN |
Cat# 40802ES03 |
| DMEM Media (High Glucose) |
HyClone |
Cat# SH30243.01 |
| RPMI Medium Modified (1640) |
HyClone |
Cat# SH30809.01B |
| PBS |
HyClone |
Cat# SH30013.04 |
| Opti-MEM |
Invitrogen |
Cat# 31985-070 |
| Hexadimethrine Bromide |
Sigma-Aldrich |
Cat# H9268 |
| Poly-D-Lysine |
Huaweiruike Chemical Co., Ltd |
Cat# HWG24863 |
| Sodium Pentobarbital |
Sigma-Aldrich |
Cat# P3761 |
| Protease/phosphatase Inhibitor Cocktail |
Cell Signaling Technology |
Cat# 5872 |
| Super ECL Detection Reagent |
Shanghai YEASEN |
Cat# 36208ES60 |
| Resazurin |
Sigma-Aldrich |
Cat# R7017 |
| Diaphorase |
Aladdin |
Cat# D128545 |
| Phosphoenolpyruvic Acid Monopotassium Salt |
Alfa Aesar |
Cat# B20358.06 |
| Phenazine Methosulfate |
Macklin |
Cat# P815754 |
| Thiazolyl Blue Tetrazolium Bromide |
Aladdin |
Cat# T100896 |
| DAF-FM DA Probe |
Beyotime Biotechnology |
Cat# S0019 |
| Fetal Bovine Serum (FBS) |
Biological Industries |
Cat# 04-001-1ACS |
| Trypsin-EDTA |
Gibco |
Cat# 25200-072 |
| Human Fibronectin |
Shanghai YEASEN |
Cat# 40105ES08 |
| Lactate Dehydrogenase |
Aladdin |
Cat# L128329 |
| Pyruvate Kinase |
Sigma-Aldrich |
Cat# P1506 |
| Alcohol Dehydrogenase |
Aladdin |
Cat# A124756 |
| Critical Commercial Assays |
| Cell Counting Kit-8 (CCK-8) |
Shanghai YEASEN |
Cat# 40203ES60 |
| Mouse IL-6 ELISA Kit |
R&D Systems |
Cat# VAL604 |
| Mouse TNF-α ELISA Kit |
Abbkine |
Cat# KET7015 |
| CellTiter-Glo® Luminescent Cell Viability Assay Kit |
Promega |
Cat# G7573 |
| Senescence β-Galactosidase Staining Kit |
Beyotime Biotechnology |
Cat# C0602 |
| Experimental Models: Cell Lines |
| HEK293T |
Cell Bank of Chinese Academy of Science |
GNHu17 |
| HEK293 |
Cell Bank of Chinese Academy of Science |
GNHu43 |
| RAW264.7 Mouse Macrophages |
Cell Bank of Chinese Academy of Science |
TCM13 |
| Human Fibroblasts CCC-HPF-1 |
Laboratory Animal Center of Hangzhou Normal University |
N/A |
| Experimental Models: Organisms/Strains |
| C57BL/6J Mice |
Laboratory Animal Center of Hangzhou Normal University |
N/A |
| Kunming Mice |
Jiesijie Experimental Animal Co., Ltd |
N/A |
| The Third Generation of Telomerase-dysfunctional Mice (refer as ‘G3Terc−/−’ or ‘G3’) |
from Prof. Lenhard K. Rudolph |
N/A |
| Zebrafish Larvae |
Zebrafish core facility, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences |
N/A |
| Oligonucleotides |
| Primer sequences for cloning or sequencing, see Table S1
|
This paper |
N/A |
| Recombinant DNA |
| pcDNA3.1-FiNad |
This paper |
N/A |
| pcDNA3.1-mCherry-FiNad |
This paper |
N/A |
| pcDNA3.1-mCherry-cpYFP |
This paper |
N/A |
| pLVX-mCherry-FiNad |
This paper |
N/A |
| pLVX-mCherry-cpYFP |
This paper |
N/A |
| pcDNA3.1-SoNar |
Zhao et al., 2015 Cell Metabolism |
N/A |
| pcDNA3.1-cpYFP |
Zhao et al., 2015 Cell Metabolism |
N/A |
| pcDNA3.1-iNap1 |
Tao et al., 2017 Nature Methods |
N/A |
| pcDNA3.1-iNapc |
Tao et al., 2017 Nature Methods |
N/A |
| pCDFDuet-mCherry-FiNad |
This paper |
N/A |
| pCDFDuet-mCherry-cpYFP |
This paper |
N/A |
| psPAX2 |
Addgene |
Cat# 12260 |
| pMD2.G |
Addgene |
Cat# 12259 |
| pCDFDuet-LigA-cpVenus |
Cambronne et al., 2016 Science |
N/A |
| pCDFDuet-cpVenus |
Cambronne et al., 2016 Science |
N/A |
| GW1-PercevalHR |
Addgene |
#49082 |
| Software and Algorithms |
| Image J 1.50d |
Fiji |
http://fiji.sc/ |
| GraphPad Prism V6.01 |
GraphPad Software |
https://download.cnet.com/s/graphpad-prism-6-01/ |
| SigmaPlot 12.0 |
SigmaPlot Software |
https://sigmaplot.software.informer.com/12.0/ |
| Gen5 1.05 |
Biotek |
https://www.biotek.com/products/software-robotics/ |
| CytExpert 2.0 |
Beckman Coulter |
https://www.beckman.com/flow-cytometry/instruments/cytoflex/software |
| NIS-Elements AR Analysis Version 5.11.01 |
Nikon |
https://www.nikon.com/products/microscope-solutions/support/download/software/imgsfw/nis-ar_v5110164.htm |
| Other |
| 35 mm Glass Bottom Dish |
Cellvis |
D35-20-1-N |
| 96-well Glass Bottom Plate |
Cellvis |
P96-1-N |
