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Plasmids used to assemble Velcro-AAV. LZ sequences were inserted after residue G453 in the AAV9 cap gene, along with spacers (red) and an enterokinase cleavage sequence (orange). For VP2-E3 and VP2-K3 constructs, the start codons for VP1 and VP3 were replaced with CTG to ablate their translation. Modified VP2-E3 and VP2-K3 plasmids were cotransfected with VP1/3 plasmid to generate mosaic capsids.
