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. Author manuscript; available in PMC: 2020 Jun 29.
Published in final edited form as: ACS Synth Biol. 2020 Feb 18;9(3):461–467. doi: 10.1021/acssynbio.9b00341

Figure 2.

Figure 2.

Characterization of Velcro-AAV vector formation and enterokinase digestion. (A) Genomic titers of 1-plate virus preps of E3 and K3 Velcro-AAV. Titers are expressed in terms of viral genomes per milliliter and were determined using qPCR. AAV9 is included as a control. N = 3 independent virus preps; error bars are SEM. One-way ANOVA was performed with Dunnett’s posthoc multiple comparison test to compare Velcro-AAV to AAV9. **p < 0.01. (B) Western blot of enterokinase (Ek)- or sham (S)-treated samples. Purified viruses were treated with enterokinase or sham, denatured, and VPs were analyzed on a Western blot using B1 antibody. B1 antibody recognizes an epitope present in the C-terminus of all VPs.