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. Author manuscript; available in PMC: 2020 Jun 29.
Published in final edited form as: ACS Synth Biol. 2020 Feb 18;9(3):461–467. doi: 10.1021/acssynbio.9b00341

Figure 3.

Figure 3.

Velcro-AAV genomic protection and transduction index after enterokinase digestion. (A) Benzonase genomic protection assay. Velcro-AAV vectors pre- and postenterokinase digestion were treated with either benzonase or sham buffer and the fraction of genomes remaining in benzonase-treated samples as compared to sham was quantified using qPCR. (B) CHO-Lec2 cells were transduced at an MOI of 5000 with Velcro-AAV. Transgene expression was measured by flow cytometry at 48 h post-transduction. Transduction index (the product of %+GFP cells and geometric mean fluorescence intensity) is reported relative to AAV9. In both panels, N = 3 experiments were conducted and error bars are SEM. One-way ANOVA was performed with Sidak’s posthoc multiple comparison test to compare Velcro-AAV to AAV9 and to compare pre- and postenterokinase digested Velcro-AAV. **p < 0.01, ***p < 0.001.