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. 2020 Jun 29;15(6):e0235215. doi: 10.1371/journal.pone.0235215

Table 3. Genotype and phenotype of 19 F10 recombinant events in the region of Pv01 used for fine mapping of the anthracnose and angular leaf spot resistance loci in CDRK.

The phenotype was obtained from the reaction of the 110 F10 RILs to races 73, 2047, and 3481 of Colletotrichum lindemuthianum and race 63–39 of P. griseola. Genotyping was achieved using the flanking markers 12 SNP, two SSR and one STS markers that enabled the positioning of the CoPv01CDRK/PhgPv01CDRK loci in a 33 kb genomic region flanked by markers CV542014 and ss715645248.

Marker SNP position Recombinant lines from CDRK × Yolano
5 12 19 20 33 38 43 47 48 62 70 73 79 87 88 91 115 96 146
ss715645260 50115685 AA AA BB BB AA BB AA AA AA BB AA AA AA BB BB AA BB AB AB
ss715645259 50130201 AA AA BB BB AA BB AA AA AA BB AA AA AA BB BB AA BB AB AB
ss715645258 50155987 AA AA BB BB AA BB AA AA AA BB AA AA AA BB BB AA BB AB AB
ss715645257 50161526 AA AA BB BB AA BB AA AA AA BB AA AA AA BB BB AA BB AB AB
ss715645256 50182775 AA AA BB BB AA BB AA AA AA BB AA AA AA BB BB AA BB AB AB
ss715645254 50203547 AA AA BB BB AA BB AA AA AA BB AA AA AA BB BB AA BB AB AB
ss715645252 50222584 AA AA BB BB AA BB AA AA AA BB AA AA AA BB BB AA BB AB AB
ss715645251 50301592 AA AA BB BB AA BB AA AA AA BB AA AA BB BB BB AA BB AB AB
BARCPVSSR01358 50350345 AA AA BB BB AA BB - AA AA BB AA AA BB BB BB AA BB BB AA
BARCPVSSR01361 50388017 AA AA BB BB AA BB - AA AA BB AA AA BB BB BB AA BB BB AA
CV542014 50513853 AA AA BB BB AA BB AA AA AA BB AA AA BB BB BB AA BB AB AB
CoPv01CDRK/PhgPv01CDRK BB AA BB BB AA BB AA AA AA BB AA AA BB BB BB AA BB BB AA
ss715645248 50546985 BB AA BB BB AA BB AA AA BB BB AA AA BB BB BB AA BB AB AB
ss715645299 51353193 BB BB AA BB AA AA AA BB BB BB BB BB BB BB BB BB AA AB AA
ss715645293 51617802 BB BB AA AA BB AA BB BB BB AA AA BB BB AA AA BB AA AA AA
ss715645250 51726047 BB BB AA AA BB AA BB BB BB AA AA BB BB AA AA BB AA BB AA
ss715645244 51764167 AA BB AA AA BB AA BB BB BB AA AA BB BB AA AA BB AA BB AA
ss715645305 51786948 AA BB AA AA BB AA BB BB BB AA AA BB BB AA AA BB AA BB AA
ss715645301 51819821 AA BB AA AA BB AA BB BB BB AA AA BB BB AA AA BB AA BB AA
ss715648967 51883712 AA BB AA AA BB AA BB BB BB AA AA BB BB AA AA BB AA BB AA
ss715648965 51896315 AA BB AA AA BB AA BB BB BB AA AA BB BB AA AA BB AA BB AA

AA = Resistant; BB = Susceptible; AB = Heterozygous;— = not available.