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. 2020 Jun 25;12:4981–4990. doi: 10.2147/CMAR.S251264

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© 2020 Yang et al.

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The association between CBLB and miR-181a-5p in EAC cells. (A) The speculative binding sites of CBLB 3ʹ-UTR and miR-181a-5p. (B) Luciferase activity was detected in EAC cells co-transfected with CBLB-WT or CBLB-MUT and NC or anti-NC, and miR-181a-5p or anti-miR-181a-5p. (C) RIP assay for the enrichment of Ago2 on miR-181a-5p and CBLB was conducted. (D) The protein expression of CBLB was examined in EAC cells transfected with NC, miR-181a-5p or anti-NC, anti-miR-181a-5p by Western blot. *P<0.05.