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. Author manuscript; available in PMC: 2020 Jun 29.
Published in final edited form as: Nat Metab. 2020 Apr 13;2(4):307–317. doi: 10.1038/s42255-020-0190-0

Extended Data Fig. 9. TGFβ-Smad3 activity in MuSCs with aging, Cyclin D1 deficiency, and pharmacologic modulation.

Extended Data Fig. 9.

a-c, Western blots on freshly isolated MuSCs to assess for activating C-terminal phosphorylation of Smad3. Each lane’s phospho-Smad3 level was normalized first to Histone 3 and then to the grand mean of each blot; blots quantified in each figure contained equals numbers of each replicate type.

a, MuSCs were from Y(Veh), O(Veh), and O(LY) mice. Shown is a representative blot and quantification of two blots. Each lane represents MuSCs from one mouse (n=6 lanes per group).

b, MuSCs were from WT(Veh), HET(Veh), and HET(LY) mice. Shown is a representative blot and quantification of two blots. Each lane represents MuSCs from one mouse (n=6 lanes per group).

c, MuSCs were from WT(Veh), KO(Veh), and KO(LY) mice. Shown is a representative blot and quantification of two blots. Each lane represents MuSCs from one mouse (n=6 lanes per group). *P<0.05; one-tailed Mann-Whitney U-test in a-e.