Fig 1. Seeded aggregation kinetics of α-syn in the presence of α-crystallin.

The aggregation kinetics of 20 μM α-syn monomer was monitored using ThT fluorescence in the presence of three different seed concentrations (A) 3 μM, (B) 1 μM and (C) 0.3 μM with α-crystallin concentrations ranging from 0.002 to 2 mg/ml in 10 mM MES buffer pH 5.5 under quiescent conditions at 37°C with color codes shown in panel C. The figures show the average trace of at least three experimental repeats. The following panels show normalized aggregation kinetic traces for 20 μM α-syn monomer in the presence of (D) 3 μM and (E) 1 μM seed with α-crystallin concentrations ranging from 0–0.02 mg/ml. The figures show the experimental repeats dotted with the fits as solid lines. (F) Fitted values of the rate constant product k₂k₊ as a function of α-crystallin concentrations.