Fig 2. Seeded aggregation kinetics of α-syn monomer in the presence of βH- and γB-crystallin.
The aggregation kinetics of 20 μM α-syn was monitored using ThT for α-syn alone (black traces) and in the presence of (A) βH- or (B) γB-crystallin at concentrations ranging from 0.002 to 2 mg/ml for one α-syn seed concentration, 1 μM, in 10 mM MES buffer pH 5.5 under quiescent conditions at 37°C. The experimental average trace of at least two repeats is shown. For stoichiometric purposes, 2 mg/ml of the high molecular weight oligomeric βH-crystallin approximately corresponds to a molar concentration of 10 μM, while for monomeric γB-crystallin it is roughly 95 μM.