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. 2020 Feb 7;26(6):809–819. doi: 10.1093/ibd/izaa015

FIGURE 7.

FIGURE 7.

Inhibition of GRP78 reduces TGFB1 transcription and TGF-β1 protein levels. A, Representative Western blot and densitometric analysis of GRP78 protein levels in SEMF of normal ileum after siRNA-mediated knockdown of GRP78 in the presence or absence of tunicamycin. B, Tunicamycin-induced increase in TGFB1 transcription is abolished by siRNA-mediated knockdown of GRP78. C, Tunicamycin-induced increase in active and total (active + latent) TGF-β1 is inhibited after siRNA-mediated knockdown of Grp78. TGFB1 transcriptional activity was measured by luciferase reporter assay. Protein levels were measured using Western blot or ELISA. Results represent the mean ± SEM of 5 to 6 experiments using normal SEMF isolated from 5 to 6 patients with fibrostenotic Crohn’s disease. *Denotes P < 0.05 between scramble siRNA and scramble siRNA + tunicamycin (TM). **Denotes P < 0.05 between scramble siRNA + tunicamycin (TM) and siRNA Grp78 + TM.