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. Author manuscript; available in PMC: 2020 Jun 29.
Published in final edited form as: J Neurosci Res. 2016 Sep 28;94(12):1434–1450. doi: 10.1002/jnr.23958

Table 2.

Primer Sequences and Sizes Used to Obtain Exon Products

Gene Primers Temp (Degrees) Size (bp)
NESTIN F-GAAGGGCAATCACAACAGGT
R-ACCCTCTTTGCTCCAAACT		 60 278
PAX6 F- AACAGGAAGGAGGGGGAGA
R-AAAACCATACCTGTATTCTTGCTTC		 60 240
OLIG2 F- TCCAATCTCAATATCTGGGTCA
R-GCTTAGAGAAGGAAAGATGAGTCG		 60 264
PDGFRa F- GAAGCTGTCAACCTGCATGA
R- GACCAAGTCCAGAATGGATGA		 60 291
SOX2 F- CATCACCCACAGCAAATGAC
R- CTTCCTGCAAAGCTCCTACC		 60 253
TF F- ACTGGATGCAGGTTTGGTGT
R- GAGAAGAAATTGGCCACTGC		 60 282
GAPDH F- GAGAGAAACCCGGGAGGCTA
R- ATGTAGTTGAGGTCAATGAAGGGG		 60 230

Human gene-specific polymerase chain reaction primers were generated from the coding sequence obtained from the National Center for Biotechnology Information. The regions of genes were selected without 3D structure using mfold software, and primers were designed using Primer3 software for 60 degrees. The selected forward and reverse primer sequence were sent to Sigma for primer synthesis. The primers were validated before study.