a, Immunohistochemical staining for phosphorylated tau (AT8) in mouse hippocampi. Scale bar, 500μm.
b, Quantification of AT8 in hippocampus and CA1 region shown in a. n=17 for Tau22, n=15 for Tau22/Asc−/−, n=8 for Tau22/Nlrp3−/−. Hippocampus: **P=0.0055, CA1: *P=0.0278, **P=0.0059.
c, Staining with thioflavine S (aggregated Tau) of mouse hippocampi. Scale bar, 500μm (top) and 100μm (bottom).
d, Quantification of thioflavine S-positive cells in CA1 region shown in c. n=12 for Tau22, n=5 for Tau22/Asc−/− and Tau22/Nlrp3−/−. Tau22 vs. Tau22/Asc−/−: *P=0.0240, Tau22 vs. Tau22/Nlrp3−/−: *P=0.0444.
e, Example of movement of mice in Morris water maze test (MWM).
f, Quantification of time spend in quadrant 1 (Q1) vs. all other quadrants (a.o.) in MWM. n=12 for WT, Nlrp3−/−, Tau22/Asc−/−, Tau22/Nlrp3−/−, n=14 for Asc−/−, n=16 for Tau22. ***P=0.0001, ****P<0.0001, Asc−/−: **P=0.0065, Nlrp3−/−: **P=0.0012. For gel source data, see Supplementary Figure 1. Data are mean ± SEM and were analyzed by one-way ANOVA with Tukey’s test (b, d) or two-tailed unpaired t-test (f).