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. Author manuscript; available in PMC: 2020 Jun 29.
Published in final edited form as: Nature. 2019 Nov 20;575(7784):669–673. doi: 10.1038/s41586-019-1769-z

Extended Data Figure 5: Knockout of Asc or Nlrp3 efficiently inhibits NLRP3 inflammasome function.

Extended Data Figure 5:

a, Immunoblot analysis of hippocampus samples from 11 month old mice stained for Caspase-1 and β-actin.

b, Quantification of data from a. n=6 for Tau22 and Tau22/Asc−/−, n=9 for Tau22/Nlrp3−/−, *P=0.0156, **P=0.0012.

c, Immunoblot analysis of hippocampus samples of 11 month old mice stained for IL-1β (p17) and β-actin.

d, Quantification of data from c. n=7 for all groups. Tau22 vs. Tau22/Asc−/−: *P=0.0399, Tau22 vs. Tau22/Nlrp3−/−: *P=0.0310.

e, Quantification of number of ASC specks/microglia, % of intracellular ASC specks and % of extracellular ASC specks in hippocampus sections of 11 month old mice. n=6 mice per group, ****P<0.0001 for all comparisons.

For gel source data, see Supplementary Figure 1. All graphs are presented as mean ± SEM and were analyzed by one-way ANOVA followed by Tukey’s test.