| Antibodies |
| Mouse monoclonal anti-AnkG |
NeuroMab Facility |
Cat#75-146; RRID: AB_10673030 |
| Goat polyclonal anti-AnkG |
Santa Cruz Biotechnology |
Cat#sc-31778; RRID: AB_2289736 |
| Rabbit polyclonal anti-Usp9X |
Abcam |
Cat#ab19879; RRID: AB_470300 |
| Mouse monoclonal anti-Flag (clone M2) |
Sigma-Aldrich |
Cat#F1804; RRID: AB_262044 |
| Mouse monoclonal anti-HA (clone HA-7) |
Sigma-Aldrich |
Cat#H3663; RRID: AB_262051 |
| Rabbit polyclonal anti-HA |
Enzo Life Sciences |
Cat#ADI-MSA-106; RRID: AB_10615792 |
| Rabbit polyclonal anti-HA |
Abcam |
Cat#ab20084; RRID: AB_445319 |
| Mouse monoclonal anti-His |
Millipore |
Cat#05-949; RRID: AB_492660 |
| Mouse monoclonal anti-β-actin (clone AC-74) |
Sigma-Aldrich |
Cat#A2228; RRID: AB_476697 |
| Rabbit polyclonal anti-TGFβRI |
Abcam |
Cat#ab31013; RRID: AB_778352 |
| Rabbit polyclonal anti-TGFβRII |
Abcam |
Cat#ab186838; RRID: AB_2728775 |
| Rabbit monoclonal anti-p-Smad2 (Ser465/467) |
Cell signaling Technology |
Cat#3101; RRID: AB_331673 |
| Mouse monoclonal anti-Smad2 |
Abcam |
Cat#ab119907; RRID: AB_10899446 |
| Rabbit polyclonal anti-phosphoserine |
Abcam |
Cat#ab9332; RRID: AB_307184 |
| Mouse monoclonal anti-Flag-488 (clone M2) |
Abcam |
Cat#ab117505; RRID: AB_10972518 |
| Rabbit polyclonal anti-HA-568 |
Synaptic System |
Cat#245 003C3; RRID: AB_2619954 |
| Chicken polyclonal anti-mCherry |
Abcam |
Cat#ab205402; RRID: AB_2722769 |
| Chicken polyclonal anti-GFP |
Abcam |
Cat#ab13970; RRID: AB_300798 |
| Rabbit polyclonal anti-GFP |
Thermo Fisher Scientific |
Cat#A10260; RRID: AB_2534022 |
| Goat anti-chicken 488 |
Thermo Fisher Scientific |
Cat#A-11039; RRID: AB_2534096 |
| Goat anti-chicken 568 |
Thermo Fisher Scientific |
Cat#A-11041; RRID: AB_2534098 |
| Donkey anti-rabbit 488 |
Thermo Fisher Scientific |
Cat#A-21206; RRID: AB_2535792 |
| Goat anti-mouse 568 |
Thermo Fisher Scientific |
Cat#A-11031; RRID: AB_144696 |
| Bacterial and Virus Strains |
|
E. coli HST08 |
TaKaRa |
Cat#636766 |
| Chemicals, Peptides, and Recombinant Proteins |
| Mouse TGFβ1 |
eBioscience |
Cat#14-8342-62 |
| Critical Commercial Assays |
| Duolink® In Situ PLA® Probe Anti-Mouse MINUS |
Sigma-Aldrich |
Cat#DUO92004 |
| Duolink® In Situ PLA® Probe Anti-Rabbit PLUS |
Sigma-Aldrich |
Cat#DUO92002 |
| Duolink® In Situ PLA® Probe Anti-Goat MINUS |
Sigma-Aldrich |
Cat#DUO92006 |
| Duolink® In Situ Detection Reagents FarRed |
Sigma-Aldrich |
Cat#DUO92013 |
| In-Fusion HD Cloning Kit |
Clontech |
Cat#638910 |
| QuickChange Site-Directed Mutagenesis Kit |
Agilent Technologies |
Cat#200516 |
| Experimental Models: Cell Lines |
| HEK293T/17 cells |
ATCC |
Cat#CRL-11268 |
| Experimental Models: Organisms/Strains |
| Mouse: C57BL/6J |
The Jackson Laboratory |
#000664 |
| Oligonucleotides |
| AnkG480 F: TGA AGG AAT TCG GTA CCA TGG CTC ATG CCG CCT CCC AGTTA |
This paper |
N/A |
| AnkG480 R: GCG GCC GCA CTC GAG CTA GTG GGC TTT CTT CTC CAC ATT CCG |
This paper |
N/A |
| Recombinant DNA |
| pEZ-3XHA-AnkG |
GeneCopoeia |
Cat# EX-Mm25668-M06 |
| pEZ-3XFlag-Usp9X |
GeneCopoeia |
Cat#EX-Mm24322-M12 |
| pRFP-C-RS Control |
Origene |
Cat#TR30015 |
| pRFP-C-RS shUsp9X |
Origene |
Cat#TF510959 |
| pGFP-C-RS shAnkG |
Smith et al., 2014 |
https://www.cell.com/neuron/abstract/S0896-6273(14)00908-8 |
| pmCherry-C1 |
Clontech |
Cat#632524 |
| pEZ-3XHA-AnkG1–807
|
Yoon et al., 2020 |
HA-AnkG1–807
|
| pEZ-3XFlag-Usp9X1555–1958
|
Yoon et al., 2020 |
Flag-Usp9XWt
|
| pEGFP-Usp9X1555–1958
|
Yoon et al., 2020 |
GFP-Usp9XWt
|
| pEZ-3XFlag-Usp9X1555–1958 S1593;1600;1609A |
Yoon et al., 2020 |
Flag-Usp9XS3A
|
| pEGFP-Usp9X1555–1958 S1593;1600;1609A |
Yoon et al., 2020 |
GFP-Usp9XS3A
|
| pMCSG53-6XHis-Usp9X1547–1962
|
Yoon et al., 2020 |
His-Usp9X1547–1962
|
| pEZ-3XHA-AnkG480 |
This paper |
HA-AnkG480 |
| Software and Algorithms |
| FIJI (ImageJ) |
NIH |
https://fiji.sc/ |
| NIS-Elements v4.51.00 |
Nikon |
https://www.nikon.com/products/microscope-solutions/support/index.htm |
| Prism 8 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
| Maestro multiple sequence viewer |
Schrödinger |
https://www.schrodinger.com/ |
| Maestro Bioluminate |
Schrödinger |
https://www.schrodinger.com/ |
| PyMOL V1.8.2.0 |
Schrödinger |
https://pymol.org/2/ |
| Image Lab 3.0 |
Bio-Rad Laboratories |
https://www.bio-rad.com/en-us/product/image-lab-software |
