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. 2020 Jun 2;130(7):3637–3653. doi: 10.1172/JCI134424

Figure 2. CRLF2 signaling is not required for survival of Ph-like ALL cells.

Figure 2

(A) MUTZ5 cells were electroporated with nontargeting control or ribonucleoproteins targeting CRLF2. CRLF2 deletion was validated via flow cytometry. (B) Western blot analysis of CRLF2+ and CRLF2-null MUTZ5 cells for the indicated proteins. (C) CRLF2-deleted (null or KO) MUTZ5 cells mixed with native CRLF2+ MUTZ5 control cells were monitored in vitro over time for outgrowth of CRLF2+ cells (n = 3 independent experiments). (D) Flow cytometry analysis of human CRLF2-deleted MUTZ5 cells in peripheral blood of engrafted NSG mice at 2 months after injection (n = 3). (E and F) End-study analysis of flow cytometry–sorted CRLF2-deleted (–) and CRLF2+ (+) MUTZ5 cells harvested from engrafted murine spleens by flow cytometry with CD19 and CRLF2/TSLPR surface staining (E) and Western blotting of the indicated proteins (F). Data are represented as individual values with mean ± SEM bars.