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. 2020 Jun 2;130(7):3621–3636. doi: 10.1172/JCI130176

Figure 5. Increased NF-κB signaling contributes to pyroptosis in nitrergic enteric neurons.

Figure 5

Increased NF-κB signaling induced pyroptosis in enteric neurons. Six-week-old IKKβ+/nNOS-CreER mice and their littermate control mice were injected with tamoxifen daily for 5 days. All the tests were performed 4 weeks following tamoxifen injection. (A) nNOS+, (B) ChAT+, and (D) CC1+ neurons per field of proximal colon. (C) Representative images of CC1, nNOS, and ChAT staining. Arrows show the colocalization of CC1 and nNOS. Scale bars: 100 μm. (E) Bead expulsion test to assess colonic motility. n = 4 per group. Data presented as the mean ± SEM. *P < 0.05; **P < 0.01 by unpaired t test with F test comparison of variances.