Skip to main content
. 2020 Jun 2;130(7):3621–3636. doi: 10.1172/JCI130176

Figure 8. Palmitate and LPS induce nitrergic neuronal degeneration in vitro, which is prevented by MβCD.

Figure 8

Enteric neurons were cultured in the presence of palmitate (0.25–0.5 mM), LPS (0.5 ng/mL), and/or MβCD for 24 hours. (A) Cytotoxicity assessed by LDH release assay. (B) Pyroptosis assessed by costaining with propidium iodide (PI) and an antibody against cleaved caspase-1 (CC1). Scale bars: 30 μm. Flow cytometry was used to analyze the percentage of (C) nNOS+ and (D) ChAT+ in neurons marked by Tuj-1. Representative flow cytometry graphs are shown. Data presented as the mean ± SEM; n = 3 mice in each group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by 1-way ANOVA with Dunnett’s multiple-comparisons test and Bartlett’s test of equal variances.