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. 2020 Jun 15;130(7):3936–3946. doi: 10.1172/JCI132438

Figure 6. Regulation of NK cell IFN-γ production by EBOV GP induced IL-10.

Figure 6

The correlation between NK cell IFN-γ secretion determined by intracellular staining and IL-10 secretion measured by Luminex in response to EBOV GP (in baseline trial samples) was determined by Spearman’s coefficient (A) (n = 70). Nonvaccinated control PBMCs were stimulated in the presence of blocking antibodies against IL-10R or isotype control (n = 16). Total NK cell IFN-γ (B), CD107a (C), and CD25 (D) expression was determined. Intracellular IL-10 was also measured by flow cytometry (gating strategy as per Supplemental Figure 5A) in B cells (CD19+), myeloid DCs (mDC; CD14–CD11c+), total CD14– and total CD14+ cells, NK cells (CD3–CD56+), and T cells (CD3+) (E). The proportion of IL-10+ events per cell type determined by back-gating is also shown as a pie chart (F). Graphs show box-and-whisker plots with median, IQR (box), and 10th to 90th percentile (whiskers). Comparisons were performed using Wilcoxon signed-rank test. **P < 0.01, ***P < 0.001, ****P < 0.0001.