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. 2020 May 26;130(7):3499–3510. doi: 10.1172/JCI134874

Figure 4. Nonbiased sequencing analyses establish the importance of Sel1L-Hrd1 ERAD in β cell identity.

Figure 4

(A and B) Results from cDNA microarray analysis of islets from 5-week-old mice (n = 3 mice each group). (A) Volcano plot depicting transcriptomics data with dotted line marking P = 0.05 on y axis and fold change of greater than 2 on x axis. (B) Heatmap showing log-fold change of mature β cell markers and forbidden genes. (C–E) Results from scRNA-Seq of islets from 7-week-old male mice (n = 2 mice each group). (C and D) Visualization of t-SNE plots generated by unsupervised clustering analysis presented as merged (C) or individual (D) data sets. In D, β cell population is highlighted. Each dot corresponds to a single cell. (E) Gene expression changes of representative β cell markers associated with different processes.