Figure 1. Notch1/2 expression on CD4⁺ T cells is required for AAI induction.

(A) Acute HDM-driven AAI induction protocol. (B) Numbers of FSC^hiSSC^hi+CD11c⁺Siglec-F⁺ autofluorescent macrophages, FSC^intSSC^hiSiglec-F⁺ eosinophils, Ly-6G⁺ neutrophils, CD19⁺ B cells, CD3⁺CD4⁺ T cells, and CD11c⁺MHCII^hi DCs in BAL fluid of PBS- or HDM-sensitized mice. (C) Intracellular flow cytometry quantification of the numbers of CD3⁺CD4⁺ T cells in BAL fluid expressing the indicated cytokines. (D and E) Flow cytometric RORγt/Gata3 profile in CD3⁺CD4⁺ T cells in BAL from HDM-treated mice (D) and quantification of Gata3⁺ cell numbers (left) and Gata3 mean fluorescence intensity (MFI) in Gata3⁺ T cells (right) (E). (F) Cytokine production in vitro by HDM-restimulated MedLN cells, measured by ELISA. (G) HDM-specific IgG1 and IgE levels in serum, determined by ELISA. Data are shown as individual values from 3–16 mice per group, together with the mean ± SEM, and are combined from 2 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 by Kruskal-Wallis test.