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. 2020 Feb 7;61(2):6. doi: 10.1167/iovs.61.2.6

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Copyright 2020 The Authors

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Figure 2. — MSC conditioned medium treatment of heterogenous retinal cultures. Heterogenous adult rat retinal cultures were left for three days either untreated (negative control; A), treated with CNTF (positive control; B), treated with MSC conditioned medium (C), treated with primed MSC conditioned medium without (D) or with etanercept (TNFα blocker) added during priming, or treated with TNFα primed MSC conditioned medium without or with etanercept added during priming (scale bar: 50 µm). The number of RGCs (E), denoted by their asymmetrical βIII-tubulin staining, and the number of RGCs with neurites (F) were quantified (n = 3). Asterisks indicate a significant increase in RGC neuroprotection/neuritogenesis in comparison to untreated control/unprimed MSC conditioned medium (P < 0.05) and includes CNTF treatment and primed MSC (via retinal medium or TNFα) conditioned medium treatment.