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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Immunol. 2020 Mar 20;204(9):2360–2373. doi: 10.4049/jimmunol.2000028

Figure 2. CopaE241K/+ mice have spontaneous activation of cytokine-secreting T cells.

Figure 2.

A. Representative flow plots of CD62L versus CD44 expression on splenic CD4+ and CD8+ T cells from Copa+/+ (WT) and CopaE241K/+ (HET) mice.

B. Percentages of naïve and memory CD4+ (left) and CD8+ T (right) cells from indicated mice (3-month-old littermates: WT, n = 8; HET, n = 7).

C. Representative flow plots of intracellular cytokine levels in splenic CD4+ (top: IFNγ, IL17A and IL13) and CD8+ T cells (bottom: IFNγ and TNFα) after PMA/Ionomycin stimulation.

D. left: Percentages of IFNγ, IL17A and IL13 producing CD4+ T cells among total CD4+ T cells from indicated mice (3-month-old littermates: WT, n = 6; HET, n = 4). right: Percentages of IFNγ and TNFα- producing CD8+ T cells among total CD8+ T cells (3-month-old littermates: WT, n = 4; HET, n = 6)

Data are mean ± SD. Unpaired, parametric, two-tailed Student’s t-test was used for statistical analysis. p < 0.05 is considered statistically significant. ns: not significant.