Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 25;37(3):89–94. doi: 10.1007/s10014-020-00365-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Figure. 3 — Molecular genetic findings. FISH-analysis showed retained 1p (a) and 19q (b) expression. Mutation analysis using Sanger sequencing showed a H3F3A K27M mutation (c) and a TERT C228T promoter mutation (d), NGS showed a PDGFRα Y849C mutation (e). Further molecular analysis showed wild-type status of all other 51 genes including BRAF, HIST1H3B, HIST1H3C, IDH1, and IDH2 status (f). Epigenome-wide methylation profiling using the Illumina EPIC Array at the Department of Neuropathology, University Hospital Heidelberg/German Cancer Research Center, allocated the tumor to the methylation class of diffuse midline glioma H3 K27M mutant (g). Magnification: a, b: 100 × oil immersion. c–e: mutations are indicated using “*”. f: wild-type gene status is indicated by green, mutant gene status by red color