Figure 1.

c-Fos is expressed in primary rat hippocampal neuronal cultures. A, rat primary hippocampal neurons were fixed at different times of culture and immunostained with an anti-c-Fos antibody (red). Scale bar, 20 μm. B, Western blotting of hippocampal cell lysates harvested at different times of culture. Membranes were immunostained with an anti c-Fos antibody (top) and an anti-tubulin (Tub) antibody (bottom) as a loading control and secondary antibodies suitable for near-IR fluorescence. The obtained images were converted to gray scale and inverted. The results of one of three independent experiments are shown.