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Stimulation of acetylation of histones H3 and H4 by polyamines. FM3A cells were cultured in the presence and absence of 50 μm DFMO for 72 h. Nucleus and cytoplasm fractions were prepared, and activity of histone acetyltransferases (HATs) was measured as described under “Experimental Procedures.” (A) Activity of histone acetyltransferases. (B) Levels of histones and their acetylation. * p < 0.05; ** p < 0.01.
