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Comparison of polyamine stimulation of histone acetylation at each lysine residue in histones H3 and H4. (A) Acetylation sites of histones H3 and H4 by Gcn5 and Hat1 (17). (B) Level of acetylation of H3 and H4. Histones were isolated from control and DFMO-treated, polyamine-reduced FM3A cells and analyzed by Western blotting using site-specific acetyl lysine antibodies as described under “Experimental Procedures.” * p < 0.05; ** p < 0.01.
