Figure 4.
Stimulation of the synthesis of several mRNAs by polyamines through stimulation of acetylation of lysine residues in histones H3 and H4. (A) mRNA levels of cell growth-related genes, Ki-67, Lyar, Pcna, and MCM-2 and of PRL30 as a control. mRNA was isolated from control and DFMO-treated FM3A cells, and cDNA was synthesized and analyzed by quantitative real-time PCR as described under “Experimental Procedures.” * p < 0.05; ** p < 0.01. (B) Analysis of active promoter regions. The ChIP assay was carried out using anti-site-specific acetyl lysine of histones H3 and H4, and promoter regions of cell growth related genes, Ki-67, Lyar, Pcna, and MCM-2, and PRL30 as a control were amplified. Experiments were repeated twice, and the mean ratio (control/DFMO-treated) is shown. More than 1.5 is shown in red.