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Identification of polyamine acting sites on Gcn5 and Hat1 mRNAs. WT and 5′-UTR-deleted Gcn5-EGFP and Hat1-EGFP fusion plasmids were transfected into NIH3T3 cells and cultured in the presence and absence of 500 μm DFMO for 72 h. Protein levels were analyzed by Western blotting using anti-EGFP antibody (Clontech). ** p < 0.01; *** p < 0.001.
