Figure 7.

Effect of miR-7648-5p on polyamine stimulation of Gcn5 synthesis. (A) MicroRNA expression plasmid pCMV-MIR7648 and its vector were transfected into NIH3T3, and the effect of polyamines was examined using DFMO-treated and untreated cells. (B) MicroRNA expression plasmid pCMV-MIR7648 and its vector were cotransfected with pGcn5-EGFP into NIH3T3 cells. Protein levels of Gcn5 and Gcn5-EGFP were determined by Western blotting as described under “Experimental Procedures” using 20 μg of cell lysate, and the ratio of control/DFMO-treated cells was shown. *** p < 0.001.