Figure 3.

Heat map of differentially expressed genes in BHT-101 ASH1L KO cell lines versus WT cells. CRISPR-Cas9 was used to create 4 cell lines with premature stop codons in different positions within ASH1L prior to the catalytic domain. RNA expression of these 4 KO cell lines was compared with that of 3 separate passages of BHT-101 WT cells. Differentially expressed genes are defined as those with FDR of <0.05 and absolute FC of >2 in all 4 KO cell lines compared with WT cells, resulting in 51 down-regulated genes and 103 induced genes in the KO cells.