P2 stability and folding.
A, melting curves for wtP2 and all studied mutants. The outliers are P38G (red) and R30Q (blue). Thick black line, wtP2. B, conformation of wtP2 and selected mutants in the presence (solid lines) and absence (dotted lines) of DMPC/DPC bicelles. C, WT P2 in water (red dashed line), 9:1 DMPC/DMPG (thin black line), and 1:1 DMPC/DMPG (thick black line). D, WT P2 in water (red dashed line) and lipid/DPC bicelles containing DMPC (black dashed line), 9:1 DMPC/DMPG (thin black line), 4:1 DMPC/DMPG (medium black line), and 1:1 DMPC/DMPG (thick black line).