Figure 4.

Inhibitors for sucrose hydrolysis by BmSUH. A–C, chemical structures of DNJ (A), DAB (B), and ACR (C). D–F, Lineweaver–Burk plots of BmSUH activity toward sucrose in the presence of DNJ (D), DAB (E), or ACR (F). Concentrations of inhibitors were as follows: 0 (open square), 0.5 (gray square), and 1.0 mm (black square) for DNJ and ACR; 0 (open square), 0.1 (gray square), and 0.5 mm (black square) for DAB. G–I, structures of the active sites in BmSUH-DNJ (G), BmSUH-DAB (H), and BmSUH-ACR (I) complexes. Colors are used in the same manner as in Fig. 2, and DNJ, DAB, and ACR are shown in magenta, brown, and orange, respectively.