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. 2020 Jun 23;11:411. doi: 10.3389/fendo.2020.00411

Figure 1.

Figure 1

Relative sensitivity of pancreatic cancer cell lines PANC-1, MIAPaCa-2 and BxPC-3 to gemcitabine treatment. The number of viable cells was determined by the trypan blue dye exclusion test. (A) Cells were treated with increasing concentrations of gemcitabine (from 20 to 2,000 μM) for 24 h. BxPC-3 cells are sensitive to all gemcitabine concentrations tested, and PANC-1 and MIAPaCa-2 cells are sensitive from 200 μM gemcitabine. (B) Cells were treated with 20 μM gemcitabine and cell viability was determined at 0, 12, 24, 48, and 72 h. There were no significant differences within 72 h of treatment in PANC-1 cells, and within 48 h of treatment in MIAPaCa-2 cells. However, a significant reduction in cell viability was observed in BxPC-3 cells from 12 h of treatment. Results are presented as percentage of viable cells compared to the untreated control (mean ± SE; n = 3). *p < 0.05 vs. untreated cells, **p < 0.01 vs. untreated cells.