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. 2020 Jun 29;10:10555. doi: 10.1038/s41598-020-67550-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Golgi dispersal is independent of apoptosis. (A) Representative immunofluorescence images of TGN38 Golgi protein and cleaved-caspase 3 in control, 5-FU and doxorubicin-treated HCT-15 cells. Samples were incubated with DAPI (blue channel), anti-TGN38 (green channel) and anti-cleaved-caspase-3 (red channel) antibodies. (B) Relative quantification of the Golgi area as showed in panel A. (C) Cropped images from immunoblots using antibodies against TGN38 in control, scramble, and miR-3135b transfected cells. GADPH was used as endogenous loading control. Complete developed Western blot membranes are provided in Supplementary data 1. Error bars represent the standard deviation of triplicate experiments and p values < 0.05 were considered to be statistically significant. NS = non-significant.