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. 2020 Jun 29;10:10552. doi: 10.1038/s41598-020-67440-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of clobetasol (DC) application in vivo or incubation of B. afzelii NE4049-infected skin with cortisone (C) in vitro at day 40 of inoculation on the lower back of mice. Infected skins (3 biopsy punches per mouse) were incubated in BSK-H complete medium at 33 °C for 11 and 19 days and: cultured directly for control (infected but non-reactivated mouse), reactivated in vivo by skin application of clobetasol (DC) and cultured, or cultured directly with cortisone included in culture medium. Borreliae were counted by dark-field microscopy using a Petroff-Hausser chamber at two time points.