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. 2020 Jun 29;10:10576. doi: 10.1038/s41598-020-67522-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phage selection of hydroxyapatite binding peptides from a randomized 8-mer peptide phage library. (a) Ratio of output/input phages after one, two, three, and four rounds of selection against hydroxyapatite. (b) The ratio of the output/input phage of hydroxyapatite-specific positive clones after the fourth biopanning.