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. 2020 Jun 29;10:10515. doi: 10.1038/s41598-020-65580-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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G-CSF increased the number of MSCs in BLM-treated lungs and promoted BMSC homing to lung tissues. (A) FCM gating strategy for the surface expression of CD31^-CD34^-CD29⁺CD44⁺Sca-1⁺ cells from mouse lungs. (B) The number of CD31^-CD34^-CD29⁺CD44⁺Sca-1⁺ cells in the lungs was analysed by FCM. (C) Identification of BMSCs in mice by FCM. Representative flow cytometric analysis of BMSCs isolated from the bone marrow and stained with antibodies against CD29, CD31, CD34, CD44 and Sca-1. (D) Representative FCM analysis of the GFP-labelled BMSC population. GFP-labelled BMSCs were infused into mice, and after 36 hours, the lungs were prepared into a single-cell suspension and analysed by FCM. (E) Data analysis of the GFP-labelled BMSC population tested by FCM. Control (BMSC) and BLM (BMSC) represent conditions where BMSCs were not pretreated before being infused into control or BLM mice, whereas control (G-CSF > BMSC) and BLM (G-CSF > BMSC) represent conditions where BMSCs were pretreated with G-CSF (30 ng/mL) before being infused into control or BLM mice. n = 3–5. *P < 0.05, **P < 0.01 between the indicated groups. Bars: mean ± SD.