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. 2020 Jun 29;10:10515. doi: 10.1038/s41598-020-65580-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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G-CSF preconditioning promoted BMSC migration via the SDF-1/CXCR4 axis in vitro. (A) The expression levels of VLA-4, ICAM-1, VCAM-1 and CXCR7 were quantified by real-time PCR. (B) The expression of CXCR4 was quantified by real-time PCR and FCM at 24 hours after G-CSF treatment. (C) The proliferation of BMSCs with or without G-CSF (30 ng/mL) was tested by CCK-8 assay. (D) Experimental design of the transwell assay: lungs collected from control and BLM mice were cut into fragments and placed in the lower transwell chamber. In the upper compartment of the transwell chamber, GFP-labelled BMSCs with or without G-CSF (30 ng/mL) pretreatment were seeded to test chemoattraction. (E) Representative image of migrated GFP-labelled BMSCs (green). (F) Analysis of GFP-labelled BMSC migration. (G) The mRNA and protein expression of SDF-1 in control and BLM-treated lungs as quantified by real-time PCR and Western blotting. n = 3. *P < 0.05, **P < 0.01 between the indicated groups. Bars: mean ± SD.