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. 2020 Jun 23;7:312. doi: 10.3389/fvets.2020.00312

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Copyright © 2020 Spada, Perego, Baggiani and Proverbio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Example of samples with gel agglutination mixed-field pattern results. Sample n. 21 showed a mixed-field pattern (identified as “misto” in the Figure) in B column and was classified as A blood type based on comparison with agglutination on tube technique, the gold standard technique. Sample n. 20 showed agglutination in both A and B columns and was AB blood type, as confirmed by agglutination on tube technique. Control columns (c) containing only PBS are included and show negative test (absence of autoagglutination).