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Cell staining and transfection efficiency detection: (A) 24-h Giemsa staining (100×); (B) 48-h Giemsa staining (100×); (C) 72-h Giemsa staining (100×); (D) expression of gga-miR-454 after cell transfection of inhibitor; (E) expression of gga-miR-454 after cell transfection of mimic; (F) expression of gga-miR-454 during the growth of chicken primary myoblasts.
