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. 2020 Jun 23;8:477. doi: 10.3389/fcell.2020.00477

FIGURE 2.

FIGURE 2

BTNs and the FGF signaling pathway. (a) Two-color in situ hybridization at 7 hpf showing Fgf8/17/18 and Neurog expression in pBTN lineage (magnified inset). (b) Same embryo as in (a), but viewed at a more dorsal focal plane, showing Neurog expression in the aBTN lineage and relative position of the aBTNs and Fgf8/17/18 expression in the tail tip (magnified inset). (c) Two-color in situ hybridization at 8 hpf showing migrating aBTN and pBTN cells on one side of the embryo. (d) Immunohistochemical staining for phosphorylated ERK (dpERK, magenta) in a 7 hpf embryo, showing posterior-to-anterior gradient in the aBTN lineage (magnified inset). aBTN lineage is labeled with Neurog[BTN] > H2B:GFP reporter plasmid expression (green nuclei). (e) Embryo electroporated with Fgf8/17/18 > Fgf8/17/18:GFP plasmid, showing Fgf8/17/18:GFP (green) emanating from the tail tip cells, spreading around the tip of the notochord. MG: Motor Ganglion. Not.: Notochord.