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. 2020 Jun 23;8:477. doi: 10.3389/fcell.2020.00477

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Copyright © 2020 Kim, Gibboney, Razy-Krajka, Lowe, Wang and Stolfi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPR/Cas9-mediated knockout of Neurog. (a) Tissue-specific (using Fog > Cas9) CRISPR/Cas9-mediated knockout of Neurog, using Asic > Unc-76:GFP as a reporter. Only 15/50 of Fog > H2B:mCherry + embryos also showed Asic > Unc-76:GFP-expressing BTNs. Open arrowhead indicates BTN with very faint reporter expression. (b) Control CRISPR embryo, showing Asic > Unc-76:GFP-expressing BTNs (white arrowheads). 42/50 of Fog > H2B:mCherry + control embryos had Asic > Unc-76:GFP-expressing BTNs. (c) Same conditions as in (a) but using GAD > Unc-76:GFP as an aBTN marker. Only 12/50 Fog > H2B:mCherry + embryos also showed GAD > Unc-76:GFP-expressing aBTNs. (d) Control CRISPR embryo, showing GAD > Unc-76:GFP-expressing aBTNs (white arrowheads). 39/50 of Fog > H2B:mCherry + control embryos had GAD > Unc-76:GFP-expressing aBTNs.