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. 2020 Jun 23;8:477. doi: 10.3389/fcell.2020.00477

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Copyright © 2020 Kim, Gibboney, Razy-Krajka, Lowe, Wang and Stolfi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of candidate effectors of BTN cell behaviors. (a) Embryo electroporated with Ebf > DcxΔC:GFP and Ebf > Unc-76:mCherry plasmids, showing GFP labeling of multiple microtubule bundles in the leading edge (the presumptive proximal process) of migrating BTNs. Insets magnified in bottom subpanels. (b) Embryo electroporated as in (a) but imaged at a later time point (12 hpf), showing a GFP-labeled microtubule bundle extending through proximal and distal processes (anterior is to the left). (c) GFP:Saxo (green) labeling putative centrioles/centrosome in a BTN cell. Nucleus labeled by H2B:mCherry (magenta). (d) GFP:Saxo is also seen in the cilia of ependymal cells of the neural tube/nerve cord. (e) Gαi-related:GFP (green) is enriched at the plasma membrane and around the Golgi apparatus, which is co-labeled with GalNAcT:mCherry (magenta).