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. 2020 Jun 23;10:1013. doi: 10.3389/fonc.2020.01013

Figure 3.

Figure 3

Effect of miR-622 overexpression on the phenotype of PDAC cells. Panc-1 cells were transfected with 12.5 nM miR-622 or the control mimic. (A,B) After 24, 48, 72, and 96 h, cell proliferation was assessed by cell counting using trypan blue (A) and cell viability was investigated by MTS assay (B). (C) After 24 h, cell invasion was examined by Transwell assay using an inverted microscope. (D) After 24 h, cell migration was examined by wound healing assay. Reference indicates the image at time zero. Bars are the means ± SEM of three independent experiments. *P < 0.05.