Figure 4.

Effect of miR-622 inhibition on EMT in PDAC cells. Panc-1 cells were transfected with 12.5 nM miR-622 or a control inhibitor. (A) After 48 h, RNA was extracted and expression of HULC, E-cadherin, N-cadherin, vimentin, and Snail was analyzed by qRT-PCR. Bars are the means ± SEM of three independent experiments. *P < 0.05. (B) After 72 h, protein was extracted, and western blotting for E-cadherin, N-adherin, vimentin, and Snail was performed. Protein levels were normalized to that of β-actin.