FIGURE 3.

Changes in abundance of CBC enzymes in SBP1-overexpressing lines versus the UVM4 recipient strain. The abundance of the CBC enzymes in transformants HA5 and HA11, generated with the SBP1-3xHA construct, and in transformants St1 and St12, generated with the SBP1-mStop construct, was determined using the QconCAT strategy. Abundances relative to those in the UVM4 recipient strain were log₂ transformed and plotted. Asterisks designate significant differences between enzymes in the transformants versus the UVM4 strain (one-way ANOVA with Holm’s multiple comparison test, p < 0.01). PGK, phosphoglycerate kinase; GAP, glyceraldehyde-3-phosphate dehydrogenase; TPI, triose phosphate isomerase; FBA/SBA, fructose-1,6-bisphosphate aldolase/sedoheptulose-1,7-bisphosphate aldolase; FBP, fructose-1,6-bisphosphatase; TRK, transketolase; SBP, sedoheptulose-1,7-bisphosphatase; RPE, ribulose-5-phosphate 3-epimerase; RPI, ribose-5-phosphate isomerase; PRK, phosphoribulokinase; rbcL, ribulose bisphosphate carboxylase/oxygenase large subunit; RBCS, ribulose bisphosphate carboxylase/oxygenase small subunit.